Logger pro absorbance full#
Prepare a blank by filling an empty cuvette ¾ full with distilled water.Use the Spectrometer to determine the length of time it takes for the absorbance to reach zero. Add the bleach and measure the amount of time it takes for the color to disappear.Īlternatively: keep the concentration of the dye constant (make one solution and split it in half) and add different amounts of bleach (10 mL and 20 mL) to observe the affect of the bleach concentration. Place one solution on the stir plate and add the stir bar. Take 100 mL of the above solution and dilute with 100 mL to get a solution of half the concentration.ġ. Add one drop of food coloring to 300 mL waterĢ. *Located in the drawers opposite the bin storage shelvesġ. Optional: Vernier Spectrometer - requires Logger Pro software*.Stir plate (located in top drawer opposite the chemical storage cabinets or on the bench).From this, the reaction order for concentration of blue food dye is determined. Bleach is added to both samples and the time until the blue color disappears is recorded. Demonstrations › Chemical Kinetics › 14.2ġ4.2 Effect of Concentration on Rate of Reaction/Reaction Orderĭescription: Two samples of blue food dye in water, one with double the concentration of dye. Sites with practicals and activity ideas. Annenberg Media (biology teacher animations-detailed not all apply to student topics).Other sites with limited media resources. PNAS (Proceedings of National Academy of Science).Brightstorm (free online video lessons).Yellowtang (compiled list of cytology graphics and animations).Protein synthesis / Translation Protein.Wiley (excellent tutorials more slideshow than animation).Freeman (excellent graphics and animation by topic).(meta-collection of human biology animations and graphics).McGraw-Hill Animations (mutliple animations organized by chapters in biology text).HHMI animations (find "Biointeractive").Diagrams of many cellular structures and proccesses at Access Excellence.Canadian Academy of Japan (course site).Save them to easy to find location (like your desktop!).Store each data collection as a unique file name (to keep track of them for later.).It will stop automatically after the assinged collection period. Click "Start" in upper right hand corner to start data collection.This can be done before or after data collection. Popup menu give you options for axis label names. On graph, axis labels can be changed by clicking on axis labels.Change collection interval to one collection every 2 seconds (15 collections in total).Click on "data collection" in heading of Logger Pro graph.You should have 15 readings to establish a mean minimum 7 readings. Take several readings using the computer.This will zero the instrument to your standard. Give it a few seconds to "settle" then press "CAL" on the colorimeter (not the computer). Place the standard in the Colorimeter.Try each wavelength and choose the one with the highest absorbance. Therefore you need to select a different color than the one in the cuvette.If a wavelength is not recommended, consider that the color of the solution indicates the light being transmitted by the solution other colors are being absorbed (try it!).Select the wavelength closest to the recommended one.Select the wavelength: 430 nm = violet, 470 nm = blue, 565 nm = green, 635 nm = red.In most cases you need a "standard" for reference so make one and identify it in your data. Place your sample solutions into your cuvettes.They are made of polystyrene and can scratch - please do not rub them hard with paper towels, use lens paper. Rinse the cuvettes inside and outside to make sure they are clean.The Colorimeter will take about 5 minutes to warm up.
Logger pro absorbance software#
The software should automatically detect the probe and switch to a screen showing "Transmittance" in the lower left corner. Plug the Colorimeter into Go-Link! and plug Go-Link! into a USB port on your computer.Turn on your computer and open Logger Pro 3.(from the Vernier instruction booklet accompanying the instrument): (cuvettes with lids-should be in with colorimeter).Basic instructions for using Vernier Colorimeter.
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